Microscropic examination of feces is a standard laboratory method for diagnosing gastrointestinal parasite infections. In North America, the ova and parasite (O&P) examination is typically performed using stool that is chemically fixed in polyvinyl alcohol (PVA) and formalin, after which the stool is concentrated by filtration to enhance sensitivity.
Read moreHospital for Tropical Diseases: Parasep vs. Ridley-Allen
The use of a concentration method by clinical laboratories is essential to increase the sensitivity of finding ova, cysts and larvae in faecal specimens as they may be too scanty to be seen by direct microscopy. The Ridley-Allen modified formol ether sedimentation technique is the method of choice for routine use by most clinical laboratories. This procedure utilises filtration of a faecal...
Read moreUniversity of Jos: Mini Parasep SF vs. Ritchie
To evaluate the efficacy of Mini Parasep® SF commercial faecal parasite concentrator for the laboratory diagnosis of intestinal parasitism. This was compared with the modified Ridley-Allen sedimentation technique.
Read moreHospital for Tropical Disease: Ridley-Allen vs. Ritchie
The simplified version of Ritchie’s formol-ether technique for the concentration of faecal cysts, ova, and larvae (Ridley and Hawgood, 1956), which is no less effective than the original, has been quite widely adopted as a useful routine diagnostic procedure.
Read moreComparison of Zinc Sulphate/Formalin Ether
Many techniques have been devised to detect helminth ova and/or protozoan cysts. These may be classified according to features of manipulation and physical attributes as follows: direct smear (wet saline mounts,with or without stains, and permanently stained mounts); sedimentation, followed by direct smear; flotation procedures, with or without centrifugation; and ether sedimentation with...
Read moreComparison of Two Parasite Concentration Techniques
Many techniques have been devised to detect helminth ova and/or protozoan cysts. These may be classified according to features of manipulation and physical attributes as follows: direct smear (wet saline mounts,with or without stains, and permanently stained mounts); sedimentation, followed by direct smear; flotation procedures, with or without centrifugation; and ether sedimentation with...
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